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首页> 外文OA文献 >ABI4 Activates DGAT1 Expression in Arabidopsis Seedlings during Nitrogen Deficiency1[C][W][OA]
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ABI4 Activates DGAT1 Expression in Arabidopsis Seedlings during Nitrogen Deficiency1[C][W][OA]

机译：氮缺乏1期间ABI4激活拟南芥幼苗中的DGAT1表达[C] [W] [OA]

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Triacylglycerol (TAG) is the major seed storage lipid and is important for biofuel and other renewable chemical uses. Acyl-coenzyme A:diacylglycerol acyltransferase1 (DGAT1) is the rate-limiting enzyme in the TAG biosynthesis pathway, but the mechanism of its regulation is unknown. Here, we show that TAG accumulation in Arabidopsis (Arabidopsis thaliana) seedlings increased significantly during nitrogen deprivation (0.1 mm nitrogen) with concomitant induction of genes involved in TAG biosynthesis and accumulation, such as DGAT1 and OLEOSIN1. Nitrogen-deficient seedlings were used to determine the key factors contributing to ectopic TAG accumulation in vegetative tissues. Under low-nitrogen conditions, the phytohormone abscisic acid plays a crucial role in promoting TAG accumulation in Arabidopsis seedlings. Yeast one-hybrid and electrophoretic mobility shift assays demonstrated that ABSCISIC ACID INSENSITIVE4 (ABI4), an important transcriptional factor in the abscisic acid signaling pathway, bound directly to the CE1-like elements (CACCG) present in DGAT1 promoters. Genetic studies also revealed that TAG accumulation and DGAT1 expression were reduced in the abi4 mutant. Taken together, our results indicate that abscisic acid signaling is part of the regulatory machinery governing TAG ectopic accumulation and that ABI4 is essential for the activation of DGAT1 in Arabidopsis seedlings during nitrogen deficiency.
机译：三酰基甘油（TAG）是主要的种子储存脂质，对于生物燃料和其他可再生化学用途非常重要。酰基辅酶A：二酰基甘油酰基转移酶1（DGAT1）是TAG生物合成途径中的限速酶，但其调控机制尚不清楚。在这里，我们显示，在氮剥夺（0.1毫米氮）期间，拟南芥（Arabidopsis thaliana）幼苗中的TAG积累显着增加，同时诱导了参与TAG生物合成和积累的基因，例如DGAT1和OLEOSIN1。缺氮幼苗用于确定导致营养组织中异位TAG积累的关键因素。在低氮条件下，植物激素脱落酸在促进拟南芥幼苗中TAG的积累中起着至关重要的作用。酵母单杂交和电泳迁移率迁移分析表明，脱落酸信号传导途径中的重要转录因子ABSCISIC ACID INSENSITIVE4（ABI4）直接与DGAT1启动子中的CE1样元件（CACCG）结合。遗传研究还表明，在abi4突变体中TAG积累和DGAT1表达降低。两者合计，我们的结果表明，脱落酸信号是控制TAG异位积累的调控机制的一部分，而ABI4对氮缺乏期间拟南芥幼苗中DGAT1的激活至关重要。

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